 Enantioselective
Reductive Amination
Recombinant
and mutagenized amine dehydrogenase has been developed with the help
of genetic modification for reductive amination of ketones.
This new enzymevariant
catalyses the reductive amination of a variety of carbonyl compounds
especially aromatic ketones to enantiopure amines.
A broad
library has been created based on the DNA-sequence of the new
enzymemutant. A team of molecular biologists and chemists of X-Zyme
conducts the screening of this library for the selection of
biocatalysts with desired properties.
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Properties
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Important
properties concerning the application of the enzyme are the
recombination and relatively high stability. Overexpression
level: 20% of total cell protein. Activity 17 U/mg. Substrate
spectra: aromatic and halogenated aliphatic ketones.
pH-optima:
7.5 (reduction).
Mg
2+ increases the activity.
1
U corresponds to the amount of enzyme which reduces 1 µmol
4-phenyl-2-butanone to 1-Methyl-3-phenylpropylamine per minute
at pH 7.5 and 30 °C
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X-Zyme
Biotechnology is a german research-based enzymes and chiral
intermediates company for the discovery and development of
innovative enzymes and chiral products.
X-Zyme’s
development program has generated an extensive portfolio of enzymes
and enzyme libraries for the synthesis of chiral intermediates and
single-enantiomer form of drugs, including chiral Alcohols, Diols
and Amins.
X-Zyme
Biotechnology provides access to unique libraries of oxidoreductases
variants and conducts the screening of these libraries for the
selection of biocatalysts with desired properties.
10
enzyme-libraries, and more than 40 Catalogue
enzymes of NAD(P)H dependent S- and R-selective
biocatalysts with a broad substrate specifity and high stability,
have been successfully overexpressed based on the patented
X-Zyme’s CTP - Technology. Additionally to the enzyme
collection and libraries, 13 patents secure a stable and innovative
technology platform for the commercial development and synthesis of
new chiral compounds
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X-Zyme
services in the field of Enzymes and chiral products comprise:
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Development
and Supply of chiral products
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Screening and creation of enzyme libraries
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Improvement and production of biocatalysts
If you are
looking for an enzyme, which is not listed, please let us know. We may be able
to screen it too. X-Zyme Biotech
will carry the risk until mutually agreed criteria are met by your
research and/or your products.
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CTP -
Technology
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13 Patents
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27 recombinant R- and S-Oxidoreductases
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Various Enzyme libraries
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Fermentors
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GC's, HPLC's
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UV-VIS-Spectrometry
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IR-Spectrometer
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Molecular biology Labs, S1
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Whole - Cell biotransformation
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Purification and Analysis Labs
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Cultivation Labs
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Reactors 100-1000 Liters
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Commercial and large scale production of
chiral compounds
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