Alcohol dehydrogenase

Biocatalysis breakthrough: new potential in chiral Intermediates

Products: Enzymes

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Carbonyl reductases ---Alcohol Dehydrogenases

Product

Cat. No.

Product

Cat. No.

R-Alcohol dehydrogenases:

Synthesis of enantiomerically pure alcohols with R-configuration. Very broad substrate specificity: aliphatic, cyclic and aromatic ketones, diketones and keto acid esters

R1-alcohol dehydrogenase

Lactobacillus brevis

R-Alcohol: NADP oxidoreductase

A-1

R2-alcohol dehydrogenase

Lactobacillus sp.

R-Alcohol: NADP oxidoreductase

A-2

R3X-alcohol dehydrogenase

Mutant of R1

accepts new substrates such as

1-cyclohexylethanone

2-acetylcyclohexanone

R4X-alcohol dehydrogenase

Mutant of R1

Synthesis of enantiomerically pure 3-buten-2-ol

3-Penten-2-ol

S-Alcohol dehydrogenases:

Synthesis of enantiomerically pure alcohols with (S)-configuration. aliphatic ketones, Ketones with bulky side chains such as acetophenone, ring-halogenated acetophenones or pinacoline.

S1-alcohol dehydrogenase

Candida sp.

S-Alcohol: NAD oxidoreductase

acepts diketones, 2,3-hexandione, short chain aliphatic ketones

A-3

S2-alcohol dehydrogenase

Candida sp.

S-Alcohol: NAD oxidoreductase

accepts, ketoesters,bulky side chains, 4-halo-3-hydroxy-butanoate

A-4

S3-alcohol dehydrogenase

Rhodococcus sp.

S-Alcohol: NAD oxidoreductase

substrates: ring-halogenated acetophenones, ketoesters

A-6

S4-alcohol dehydrogenase

Thermoanaerobium sp.

S-Alcohol: NADP oxidoreductase

short and medium chain aliphatic ketones

A-8

S5-alcohol dehydrogenase (New)

Horse liver

S-Alcohol: NAD oxidoreductase

accepts aromatic aldehydes

A-9

S6-alcohol dehydrogenase(New)

Rhodococcus sp.

S-Alcohol: NAD oxido reductase

substrates: ring-halogenated acetophenones, ketoesters

A-10

S7-alcohol dehydrogenase

Thermoanaerobium sp.

S-Alcohol: NADP oxidoreductase

very stable, accepts short and medium chain aliphatic ketones

A-11

S8-alcohol dehydrogenase (New)

Thermoanaerobium sp.

S-Alcohol: NADP oxidoreductase

synthesis of aliphatic hydroxy esters

A-12

KM-alcohol dehydrogenase

Yeast

NAD oxidoreductase

A-5

SC-alcohol dehydrogenase

Yeast

NAD oxidoreductase

A-7

ADH-Kit

Lactobacillus, Candida, Rhodococcus, Thermoanaerobium

R,S-Alcohol: Various Alcohol DH

A-13

Dehydrogenases

Alanine dehydrogenase

Bacillus sp.

L-Alanine: NAD oxidoreductase

B-1

Diacetyle Reductase

Lactobacillus sp.

Acetoin: NAD oxidoreductase

B-2

Formate dehydrogenase

Candida sp.

Formate: NAD oxidoreductase

B-3

D-Glucose dehydrogenase

Bacillus sp.

D-Glucose: NADP1- oxidoreductase

B-4

D-3-Hydroxybutyrate DH

Pseudomonas sp.

R-3-Hydroxybutyrate,NAD

B-5

Hydroxysteroid dehydrogenase

Clostridium sp.

Hydroxysteroid: NADP oxidoreductase

B-6

D-Lactate dehydrogenase

Lactobacillus sp.

(R)-lactate: NAD oxidoreductase

B-7

L-Lactate dehydrogenase

Lactobacillus sp.

(S)-lactate: NAD oxidoreductase

B-8

L-Leucine dehydrogenase

Bacillus sp.

L-leucine: NAD oxidoreductase

B-9

Malate dehydrogenase

Baker’s Yeast

L-Malate: NAD⁺oxidoreductase

B-10

L-Phenylalanine dehydrogenase

Rhododcoccus sp.

L-Phenylalanine: NAD oxidoreductase

B-11

Decarboxylases

Malic decarboxylase NAD spec.

Yeast

(S)-malate:NAD oxidoreductase

C-1

Malic decarboxylase NADP Spec.

Yeast

L-Malate:NADP⁺oxidoreductase

C-2

Pyruvate decarboxylase

Yeast

Decarboxylation, Carboligation

C-3

Oxalacetate decarboxylase

Yeast

Decarboxylation, Carboligation

C-4

Lyases

Benzaldehyd-Lyase

Pseudomonas sp.

Benzoin benzaldehyde lyase

D-1

(R)-Oxynitrilase

Mandelonitrile lyase

D-2

(S)-Oxynitrilase

Acetone-cyanohydrin lyase

D-3

Oxidases

OX1-alcohol oxidase

Candida sp.

Alcohol: Oxygen oxidoreductase

E-1

OX2-alcohol Oxidase

Hansenula sp.

Alcohol: Oxygen oxidoreductase

E-2

OX3-alcohol Oxidase

Pichia sp.

Alcohol: Oxygen oxidoreductase

E-3

D-amino acid Oxidase

Microbial source

D-Amino Acid: Oxygen oxidoreductase

E-4

Sulfhydryl Oxidase (New)

Saccharomyces sp.

oxidationofthiolstodisulfides

E-5

Sulfhydryl Oxidase X1(New)

Saccharomyces sp.

Oxidation of thiols to disulfides

E-6

Transferases

Fructosyltransferase (disaccharides sucrase)

Bacillus Sp.

Production of novel disaccharides

F-1

Fructosyltransferase (Levan sucrase)

Bacillus sp.

Sucrose-6-fructosyl Transferase

F-2

PRODUCTS

BIOCATALYSTS

SYNTHESIS OF CHIRAL COMPOUNDS

CONTACT

X-Zyme GmbH
Merowingerplatz 1A
40225 Duesseldorf
Germany
E-mail
Tel: 0211-3398946
Fax: 0211-33679669

Technologies and Capabilities

· CTP - Technology

· 13 Patents

· 27 recombinant R- and S-Oxidoreductases

· Various Enzyme libraries

· Fermentors

· GC's, HPLC's

· UV-VIS-Spectrometry

· IR-Spectrometer

· Molecular biology Labs, S1

· Whole - Cell biotransformation

· Purification and Analysis Labs

· Cultivation Labs

· Reactors 100-1000 Liters

· Large Scale production of chiral compounds

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Spezialenzyme chirale Verbindungen Enzym-Membran-Reaktoren und F&E-Arbeiten auf dem Gebiet der Biokatalyse an. biotransformation chiral synthesis We offer special enzymes chiral compounds enzyme membrane and R&D services in the field of biocatalysis. Enzyme Alkohole Biotransformation Esterase asymmetrisch Formiat enzymes alcohols chiral NADH Neu5Ac dehydrogenase biotransformation esterases Formate F&E Bioprodukte Oxidoreduktasen Transferasen Lyasen Ketone Lactone Membran Reaktor Forschungszentrum Biochemie Chemie chiral NADH Neu5Ac Dehydrogenase Mikrobiologie Biotechnologie FDH Nukleotid Nukleotide Wandrey Kula Hummel enzymatisch Enantioselektivit enantiomerenrein asymmetric Biokatalysator Biokatalyse Firma optisch Reduktion Oxidation Alkohol Laktat L-Alanin Oxidase Chloroperoxidase Diacetyl Fructose Hydrolasen Glucose Glycerin Hydroxybutyrat Hydroxysteroid Laccase L-Leucin L-Phenylalanin Brenzcatechin Methyltransferase Acylase Acetamidozimts Chitinase Lipase Penicillin Peptid Amidase Tannase Aldolase Oxynitrilase Tryptophanase recombinant Tyrosinase PEG Diole (R)-5-Chlor-2-pentanol (S)-4-Chlor-3-hydroxybutyrat (R)-3-Hydroxybutyrat (S)-3-Hydroxybutyrat (R)-2-Hydroxy-4-phenylbutyrat (R)-1-Phenylethanol (S)-2-Octanol (R)-2-Octanol (R)-1 3-Butandiol (S)-1 3-Butandiol (2R 6R)-2 6-Heptandiol (2R 5R)-2 5-Hexandiol (2S 5S)-2 5-Hexandiol (3R 6R)-3 6-Octandiol (2R 4R)-2 5-Pentandiol (R)-5-Hexanolid (R)-Valerolacton (R)-2-(1-hydroxyethyl)pyridin Hexandiol Pentandiol Butandiol Heptandiol Octandiol Cofaktor Cofaktoren Glycosyltransferasen Reinigung Chromatographie Fermentation Proteine Bacillus cereus Candida boidinii parapsilosis Rhodococcus erythropolis Lactobacillus kefir brevis transformation Screening Intermediate pharmazeutisch Pharmazeutika E. coli Pichia pastoris microbiell Caldariomyces fumago Gluconobacter megaterium Pseudomonas Cellulomonas Agaricus bisporus Coriolus versicolor cellobiosus Brevibacterium Staphylococcus carnosus Manihot esculenta NAD NADP Razematspaltung R&D bioproducts oxidoreductases transferases hydrolases lyases PEG-derivatives ketones lactones membrane reactor Research Centre biochemistry chemistry microbiology biotechnology nucleotide nucleotides enzymatic enantioselectivity enantiomeric biocatalyst biocatalysis transformation screening intermediates pharmaceutical pharmaceuticals company optically optical reduction oxidation alcohol lactate L-Alanine oxidase chloroperoxidase diacetyl Glycerol hydroxybutyrate hydroxysteroid L-Leucine L-Phenylalanine catechol methyltransferase Acetamidocinnamate peptide amidase affinity gel N-Acetylneuraminic diols (R)-5-chloro-2-pentanol (S)-4-chloro-3-hydroxybutyrate Penicillium Trichoderma Rhizomucor Rhodotorula Streptomyces Thermomyces antarctica rugosa Rhizopus Aspergillus oryzae (R)-3-hydroxybutyrate (S)-3-hydroxybutyrate (R)-2-hydroxy-4-phenylbutyrate (R)-1-phenylethanol (S)-2-octanol (R)-2-octanol (R)-1 3-butanediol (S)-1 3-butanediol (2R 6R)-2 6-heptanediol (2R 5R)-2 5-hexanediol (2S 5S)-2 5-hexanediol (3R 6R)-3 6-octanediol (2R 4R)-2 5-pentanediol (R)-5-hexanolide (R)-valerolactone (R)-2-(1-hydroxyethyl)pyridine hexanediol pentanediol butanediol heptanediol octanediol cofactor cofactors sialic glycosyltransferases purification chromatography fermentation proteins Lactobacillus recombinant microbial Racemic Resolution