ELISA Mothers Against Decapentaplegic Homolog 3 (SMAD3)

Reactivity: (Homo sapiens) UniProt:P84022 Abbreviation:SMAD3 Alternative Names:DKFZp586N0721; DKFZp686J10186; HSPC193; HsT17436; JV15-2; MADH3; MGC60396; MAD; mothers against decapentaplegic homolog 3|SMA- and MAD-related protein 3|SMAD; mothers against DPP homolog 3|mad homol Application:ELISA Range:0.625-40 ng/mL Sensitivity:0.224 ng/mL Intra-AssayCV:?6.2% Inter-AssayCV:?8.3% Recovery:0.99 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate SMAD3 in samples. An antibody specific for SMAD3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySMAD3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for SMAD3 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SMAD3 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:SMAD3 or Mothers against decapentaplegic homolog 3 is a gene which appears in s on chromosome 15. It is one of several homologues of a gene which was originally discovered in the fruit fly Drosophila melanogaster. The name SMAD3 also refers to a member of the SMAD family of proteins. SMAD3 or Mothers against decapentaplegic homolog 3 is a polypeptide that, as its name describes, is a homolog of the Drosophila protein "Mothers against decapentaplegic". It belongs to the SMAD family of proteins, which belong to the TGF? superfamily of modµLators. Like many other TGF? family members, SMAD3 is involved in cell signalling. SMAD3 modµLates signals of activin and TGF?'s. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).