ELISA lexA Antibody, HRP

Quantity :50µg Clone Number: Aliases:lexA antibody; exrA antibody; spr antibody; tsl antibody; umuA antibody; b4043 antibody; JW4003LexA repressor antibody; EC 3.4.21.88 antibody Product Type:Polyclonal Antibody Immunogen Species:Escherichia coli (strain K12) UniProt ID:P0A7C2 Immunogen:Recombinant Escherichia coli LexA repressor protein (1-202AA) Raised in:Rabbit Reactivity:Escherichia coli Tested Applications:ELISA Background:Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. Binds to the 16 bp palindromic sequence 5\'-CTGTATATATATACAG-3\'. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regµLon and eventually DNA repair. Implicated in hydroxy radical-mediated cell death induced by hydroxyurea treatment (PubMed:20005847).The SOS response controls an apoptotic-like death (ALD) induced (in the absence of the mazE-mazF toxin-antitoxin modµLe) in response to DNA damaging agents that is mediated by RecA and LexA (PubMed:22412352). Clonality:Polyclonal Isotype:IgG Purification Method:>95%, Protein G purified Conjµgate:HRP Buffer:Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 Form:Liquid Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names:lexA Research Areas:Others